epcam magnetic separation 4634

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DETECTION OF CIRCULATING TUMOR CELLS BY THE CellSearch

In addition to magnetic bead/particle separation methodologies, PCR greatly facilitates the detection of occult tumor cells through the use of nucleic acid analysis. This approach is taken by the second commercially available system for detecting CTCs, developed by AdnaGen.

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Isolation of Circulating Tumor Cells in Patients with

Immunomagnetic bead separation based on antibodies for tumor cell surface antigens is currently the standard method employed to detect CTCs in the clinical setting. Epithelial cell adhesion molecule (EpCAM) is an epithelial cell–specific adhesion molecule that iswidelyexpressed on the surface of epithelial cells and epithelial-derived tumor cells (6).

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Comparison of ultracentrifugation, density gradient

including ultracentrifugation (UC-Exos), OptiPrep™ density-based separation (DG-Exos), and immunoaf-finity capture using anti-EpCAM coated magnetic beads (IAC-Exos). Notably, all isolations contained 40–100 nm vesicles, and were positive for exosome markers (Alix, TSG101, HSP70) based on electron microscopy and Western blotting.

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CST

Immunoprecipitation of EpCAM from mIMCD-3 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP Isotype Control #3900, and lane 3 is EpCAM (E6V8Y) XP Rabbit mAb (Mouse Preferred). Western blot analysis was performed using EpCAM (E6V8Y) XP Rabbit mAb (Mouse Preferred).. Learn more about how we get our images.

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Nanotechnology

Other nanomaterials for CTC detection. They used aptamer-coated NaYF4 (Yb:Er) UCNPs that targeted cancer cells and demonstrated that the fluorescence intensity was linearly correlated with the concentration of captured cancer cells, with a detection limit of 10 cancer cells in 10 ml of blood.

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Circulating tumor cell technologies

Jan 28, 2016The CellSearch system uses ferrofluid nanoparticles functionalized with an EpCAM antibody to allow magnetic separation of EpCAM+ cells from solid blood components following centrifugation. The captured cells are then immunostained to confirm the expression of CK 8, 18, 19, and DAPI, as well as the lack of CD45 expression.

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Role of circulating tumor cells in future diagnosis and

Journal of Cancer Metastasis and Treatment is an open access journal, focusing on basic and clinical studies related to cancer cell, cell biology, oncology, radiation therapy and radiology, obstetrics and gynecology, pediatrics, surgery, hematology, neuro-oncology, etc.

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rapid magnetic separator

Magnetic Separator Price, Wholesale Suppliers - Alibaba. Sponsored Listing The magnetic separator combines the characteristics of international advanced high intensity magnetic separators, which is so far the high intensity magnetic separator of best performance and most advanced technology.

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for enhanced enrichment of circulating tumor cells

Zhang et al. used a reverse protocol where anti-EpCAM beads are also added to the sample and labeled cancer cells are first enriched on a filter, and then detached from the filter using magnetic force.35 It is EpCAM expression that dictates isolation, and the cells, enlarged by the magnetic beads, are thus captured by the filter.

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Supporting Information

magnetic separation CCRF-C EM 10-3103 10 8 Fluorescence Anti-EpCAM-MNs + QDs-anti-EpCAM magnetic separation MCF-7 10-3103 5 9 Fluorescence Anti-IgG-MBs + QDs-anti-EpCAM magnetic separation SK-BR3 400-5.0104 390 10 Fluorescence Anti-EpCAM-MNs + Ag 2 S nanoassembly magnetic separation MCF-7 10-500 6 This Work

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Circulating Tumor Cells in Gastric Cancer

Circulating Tumor Cells in Gastric Cancer. According to their biology characteristics, CTCs can be subdivided into two groups: epithelial-like and EMT-associated. Epithelial-like CTCs usually form from the primary tumor and enter circulation directly by forces from external side, such as friction, mechanical forces, or tumor growth [ 2 ].

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Rapid and Sensitive Detection of Cancer Cells Based on the

Rapid and Sensitive Detection of Cancer Cells Based on the Photothermal Effect of Graphene Functionalized Magnetic Microbeads Hongyan Zhang,†,‡, Zhen Zhang,‡, Yanhong Wang,‡ Chuanchen Wu,‡ Qingling Li,† and Bo Tang*,† †College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for

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Significance of Micrometastases: Circulating Tumor Cells

the epithelial cell expression of the epithelial cell adhesion molecule (EpCAM) to enrich the sample [11]. EpCAM antibodies are attached to microscopic iron particles (ferrofluid). CTCs bind EpCAM with subsequent magnetic separation from the blood. For identification as CTCs, these cells

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Detection of Circulating Tumor Cells Using a Novel

In this article, we evaluate a new CTC separation method based on a combination of anti-EpCAM and immunomagnetic beads with the aim to detect CTCs more conveniently and specifically. METHODS: Lung cancer cells were magnetically labeled by anti-EpCAM magnetic beads, and subsequently captured by magnetic separation using our novel device.

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US6551843B1

Disclosed is a method that improves the efficiency of reactions between specific binding pairs. By translating one member of the pair through a suspension of the second member, or across a surface to which the second member is immobilized, collision and binding frequencies are greatly increased. The resulting increased binding frequency allows for reduced incubation times and lower incubation

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PlusCellect™ Human EpCAM Kit

The Human EpCAM PlusCellect Kit is designed to isolate cells via a positive selection principle. The resulting cell preparation is highly enriched for EpCAM+ cells. Purity of recovered EpCAM+ cells typically ranges between 80 - 95%. STORAGE Reagents are stable for 12 months from the date of receipt when stored in the dark at 2 - 8 C. DO NOT FREEZE. 2

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Inertial Focusing for Tumor Antigen Dependent and

clinical samples using a series of debulking, inertial focusing, and magnetic separation steps. The sorted CTCs could engineered an automated platform, called the ''CTC-iChip,'' that captured both EpCAM+ and EpCAM− cancer cells in detectable in cancers with low EpCAM expression, like triple-negative breast cancer or melanoma. Ozkumur et al.

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Circulating tumor cells: a review of Non

Apr 01, 2016In the batch separation approach, the whole labeled sample is subjected to a magnetic field at once, resulting in the migration of labeled cells to the regions of higher magnetic frequency (56). The EasySep[TM] system (STEMCELL Tech), MojoSort[TM] (Biolegend), and Dynabeads[R] (ThermoFisher) are based on this principle.

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EpCAM

In total, in 20 out of 29 EpCAM-depleted fractions (69%) from 25 metastatic breast cancer patients additional EpCAMneg CTCs could be identified [range of 1–24 CTCs per sample] applying Trop2, CD49f, c-Met, CK8 and/or HA magnetic enrichment. EpCAMneg dual-positive (CKpos/CD45pos) cells could be traced in 28 out of 29 samples [range 1–480].

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epcam magnetic separation 4634

separation followed by magnetic isolation of either CTCs by capture on anti-EpCAM magnetic beads or to preserve CTC diversity, in a negative selection mode by removing WBCs using anti-CD45 magnetic beads.33 Negative selection however is costly as large amounts of antibody-conjugated magnetic beads are needed, and some CTCs may be removed along with

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Anti

Jan 07, 2009Anti-Epithelial Cell Adhesion Molecule Antibodies and the Detection of Circulating Normal-Like Breast Tumor Cells Anieta M. Sieuwerts. (to detect epithelial cells). After incubation with staining reagents, the magnetic separation is repeated and excess staining reagents are removed by aspiration. In the final processing step, the

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MagCellectTM Plus Human EpCAM+ Cell Isolation Kit

The MagCellectTM Plus Human EpCAM+ Cell Isolation Kit is designed to isolate Epithelial Cell Adhesion Molecule (EpCAM) expressing cells via a positive selection principle. The resulting cell preparation is highly enriched with EpCAM + cells.

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Circulating Tumor Cells

A Quadrupole Magnetic Cell Sorter (QMS) was produced for the rapid enrichment and separation of CTCs from hematopoietic cells (Ikotech, Greenville, IN). QMS can sort 10 million cells per second, with a 99% depletion efficiency of CD34(+) and CD45(+) peripheral blood cells.

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Benchtop Technologies for Circulating Tumor Cells

Circulating tumor cells (CTCs) are tumor cells that have detached from primary tumor site and are transported via the circulation system. The importance of CTCs as prognostic biomarker is leveraged when multiple studies found that patient with cutoff of 5 CTCs per 7.5 mL blood has poor survival rate. Despite its clinical relevance, the isolation and characterization of CTCs can be quite

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Up

As shown in Fig. 3, the CD133 + /EpCAM + enriched cell population revealed the highest proliferation rate, measured in cells directly derived from magnetic separation (Fig. 3a) as well as in cells that, after 24 h from separation, were subjected to the xCELLigence assay (Fig. 3b, c).

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Take a closer look at exosomes

There's an urgent need for the development of new methods for vesicle isolation and characterization. To answer this need, we're continually expanding our product offerings. The products presented here can help simplify the overall study of exosomes, and are also applicable to translational research.

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Implementation of Mass Cytometry for Immunoprofiling of

2.4. Magnetic Separation of Cells. Cells labeled with antibodies conjugated to magnetic beads were separated by the autoMACS Pro Separator (Miltenyi Biotec). For disposal of dead cells from PBMCs or tumor cell suspensions, the Dead Cell Removal Kit (Miltenyi Biotec) was used.

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Use of Microfluidic Technology for Cell Separation

Use of Microfluidic Technology for Cell Separation 197 shape. The change in shape and the increased rigidity of the RBCs lead to obstruction of capillaries, thereby restricting blood flow to organs, causing anemia and other "sickle cell crisis", and decreasing life expectancy. Cancer is a second example of such changes [20].

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Take a closer look at exosomes

Human EpCam isolation 50 preps 10618D Human CD63 (isolation/detection) 30 preps 10606D Flexible streptavidin-based system (use your own biotinylated antibody) 30 preps 10608D Preenriched exosomes can be easily visualized by flow cytometry while bound to the surface of Dynabeads magnetic beads, enabling the detection of specific exosomal markers.

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Alexa Fluor 647 Conjugated Goat Anti Mouse Igg

Anti-EpCAM-MNs and Anti-FRα-MNs was added to the whole blood and after incubation, magnetic separation and fluorescence identification, the cells of DAPI+ /CK+ /CD45− were defined as CTC. Article Snippet: After thoroughly washing with PBST (3 5 min), the cells were then incubated with Alexa Fluor 647 conjugated goat anti-mouse IgG (H

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